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Eco-Friendly Multistage Treatment of Dairy Wastewater Using
Natural Coagulants: Mechanisms, Performance Evaluation, and
Future Perspectives-A Review
C.Thamaraiselvi¹, C.V. Hemalakshmi²
Department of Biotechnology, Mother Teresa Women’s University, Tamil Nadu, India
*Corresponding Author
DOI: https://doi.org/10.51583/IJLTEMAS.2026.150400115
Received: 01 May 2026; Accepted: 05 May 2026; Published: 20 May 2026
ABSTRACT
The dairy industry is one of the major agro-based sectors contributing significantly to global wastewater
generation. Dairy effluent is characterized by high concentrations of organic matter, suspended solids, fats, and
nutrients, resulting in elevated biochemical oxygen demand (BOD) and chemical oxygen demand (COD).
Conventional treatment methods using chemical coagulants such as alum and ferric salts are widely employed;
however, these methods are associated with drawbacks including high sludge production, residual toxicity, and
increased operational costs. In recent years, natural coagulants derived from plant-based materials, biopolymers,
and agro-wastes have emerged as sustainable alternatives due to their biodegradability, low toxicity, and
economic feasibility. This review critically examines the characteristics of dairy wastewater, mechanisms of
coagulation, and the performance of various natural coagulants. Furthermore, their integration into multistage
treatment systems and future prospects for large-scale applications are discussed, highlighting their potential for
sustainable wastewater management.
Keywords: Dairy wastewater, natural coagulants, sustainable treatment, coagulation-flocculation, eco-friendly
wastewater management.
INTRODUCTION
The rapid growth of the dairy industry has resulted in increased water consumption and wastewater generation.
Dairy processing units utilize large volumes of water for operations such as equipment cleaning, pasteurization,
homogenization, and product processing. Consequently, the generated wastewater contains substantial amounts
of biodegradable organic matter, including lactose, proteins, and lipids (Demirel et al., 2018; Verma et al., 2018;
Ahmad et al., 2021). In addition to organic pollutants, dairy effluents also contain detergents, sanitizers, and
inorganic salts, which contribute to fluctuating physicochemical characteristics (Kushwaha et al., 2020; Rinaudo,
2019).
The seasonal variation in milk production and processing further influences wastewater composition, making
treatment more complex (Demirel et al., 2018; Kushwaha et al., 2020).Moreover, stringent environmental
regulations necessitate the implementation of efficient and sustainable treatment strategies (Chen, 2004; Ali et
al., 2021).The discharge of untreated dairy wastewater into natural water bodies leads to severe environmental
issues such as oxygen depletion, eutrophication, and ecological imbalance (Kushwaha et al., 2020; Muthuraman
and Sasikala, 2018; Rinaudo, 2019; Abidin et al., 2020).Coagulation–flocculation is a widely used primary
treatment method (Verma et al., 2018; Yin, 2018; Bhatia et al., 2018). However, chemical coagulants raise
concerns regarding sludge toxicity and cost (Verma et al., 2018; Yin, 2018; Chen et al., 2021).
Natural coagulants have gained attention due to their eco-friendly nature (Teh et al., 2016; Choy et al., 2017;
Zaman et al., 2020; Zhou et al., 2022).
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REVIEW METHODOLOGY
This review was conducted using a systematic and structured approach to ensure scientific rigor and
transparency. Relevant literature was collected from major academic databases, including Scopus, Web of
Science, ScienceDirect, and Google Scholar.
The literature search was performed using specific keywords such as “dairy wastewater treatment,“natural
coagulants,” “coagulation–flocculation,” and eco-friendly wastewater management. Boolean operators
(AND, OR) were applied to refine the search and improve relevance.
The inclusion criteria were:
Peer-reviewed journal articles published between 2004 and 2024
Studies focusing on dairy wastewater or similar high-strength organic effluents
Research evaluating natural coagulants or comparative analysis with chemical coagulants
Studies reporting quantitative performance data such as COD, BOD, and turbidity removal
The exclusion criteria included:
Non-peer-reviewed articles, reports, and conference abstracts
Studies lacking experimental or quantitative data
Research unrelated to coagulation–flocculation or dairy wastewater treatment
After screening and eligibility assessment, relevant studies were selected and critically analyzed to compare
treatment efficiency, mechanisms, operational parameters, and limitations. The collected data were synthesized
to provide a comprehensive evaluation of natural coagulants and their applicability in multistage wastewater
treatment systems.
Characteristics of Dairy Wastewater
Dairy wastewater is a complex and highly variable effluent, the composition of which depends on processing
operations, product type, and cleaning practices. It is typically rich in biodegradable organic matter, including
lactose, proteins, fats, and oils, along with suspended solids and essential nutrients such as nitrogen and
phosphorus (Ahmad et al., 2019; Kushwaha et al., 2020; Ahmad et al., 2021). The presence of detergents,
sanitizers, and residual chemicals further contributes to fluctuations in physicochemical properties, making the
wastewater composition dynamic and difficult to standardize.
One of the defining features of dairy effluent is its high organic load. Biochemical oxygen demand (BOD) values
generally range between 800 and 3000 mg/L, while chemical oxygen demand (COD) can exceed 5000 mg/L,
indicating a substantial concentration of biodegradable pollutants (Demirel et al., 2018; Kushwaha et al., 2020;
Ahmad et al., 2021). This elevated organic content promotes rapid microbial activity, which can significantly
deplete dissolved oxygen in receiving water bodies if discharged untreated.
In addition, fats and oils present in the wastewater tend to form stable emulsions and surface films, which hinder
oxygen transfer and reduce the efficiency of biological treatment processes (Muthuraman and Sasikala, 2018;
Rinaudo, 2019). Proteins such as casein and whey contribute to the colloidal nature of the effluent, making solid
liquid separation challenging without adequate pre-treatment. Furthermore, dissolved solids, including chlorides,
sulfates, and residual cleaning agents, may interfere with downstream biological processes and affect overall
treatment performance (Ahmad et al., 2021; Ali et al., 2021).
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Another critical aspect is the variability in wastewater characteristics due to seasonal production changes and
fluctuations in water usage. Hydraulic and shock load variations in dairy industries can significantly impact
treatment system stability and efficiency. These factors necessitate the adoption of robust and adaptable treatment
strategies, particularly effective pre-treatment methods such as coagulation–flocculation, to destabilize colloidal
particles and enhance pollutant removal.
Table 1. Typical Characteristics of Dairy Wastewater
S.NO
PARAMETER
RANGE
1.
pH
4.5-8.5
2.
BOD (mg/L)
800-3000
3.
COD(mg/L)
1500-5000
4.
TSS(mg/L)
200-1000
5.
Oil & Grease (mg/L)
50-500
Environmental Impact of Dairy Wastewater
The discharge of untreated dairy wastewater poses significant environmental challenges due to its high organic
and nutrient content. Elevated levels of biodegradable organic matter stimulate rapid microbial growth, leading
to the depletion of dissolved oxygen in receiving water bodies. This results in hypoxic or anaerobic conditions
that are detrimental to aquatic organisms (Kushwaha et al., 2020; Rinaudo, 2019; Ahmad et al., 2021).
In addition to oxygen depletion, the presence of nutrients such as nitrogen and phosphorus contributes to
eutrophication, promoting excessive algal growth and subsequent ecosystem imbalance (Rinaudo, 2019; Crini
et al., 2018). The decay of organic matter further generates unpleasant odors and releases greenhouse gases,
including methane and carbon dioxide, thereby contributing to climate-related impacts (Chen, 2004; Mohd
Salleh et al., 2019).
Another critical concern is the potential contamination of soil and groundwater resulting from improper disposal
practices. The infiltration of untreated effluent can degrade water quality and pose risks to human health.
Furthermore, the presence of residual detergents and cleaning agents may introduce toxic effects on aquatic and
terrestrial ecosystems.
These environmental implications highlight the necessity for efficient and sustainable treatment strategies,
particularly those that minimize ecological impact while maintaining high treatment efficiency (Ali et al., 2021).
Conventional Chemical Coagulants: Performance and Limitations
Chemical coagulants such as alum and ferric salts are widely employed in wastewater treatment due to their high
efficiency in removing turbidity, suspended solids, and colloidal particles. These coagulants function primarily
through charge neutralization and sweep flocculation mechanisms, facilitating the aggregation and subsequent
settling of suspended particles (Verma et al., 2018; Yin, 2018). Their effectiveness, reliability, and ease of
application have made them the preferred choice in many industrial treatment systems.
Despite these advantages, several limitations restrict their long-term sustainability. One of the major concerns is
the generation of large volumes of sludge, which often contains residual metal ions and requires careful handling
and disposal. This not only increases operational costs but also raises environmental concerns. Additionally, the
performance of chemical coagulants is highly sensitive to pH and dosage conditions, necessitating continuous
monitoring and process optimization.
Another significant drawback is the potential health risk associated with residual metal content in treated water
if not adequately controlled. Furthermore, the reliance on chemical inputs contributes to increased treatment
costs and environmental burden. These limitations have driven growing interest in alternative approaches,
particularly the use of natural coagulants, which offer a more sustainable and eco-friendly solution.
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Natural Coagulants: Mechanisms and Performance
Natural coagulants are derived from plant materials, animal sources, and microorganisms.
Natural coagulants have emerged as promising alternatives to conventional chemical coagulants due to their
biodegradability, low toxicity, and sustainable origin. These coagulants are typically derived from plant
materials, animal sources, and microbial products, and they contain bioactive compounds such as proteins and
polysaccharides that facilitate the coagulation process (Teh et al., 2016; Choy et al., 2017; Zaman et al., 2020;
Mehta et al., 2021).
Protein-based coagulants, such as those obtained from Moringa oleifera, function primarily through charge
neutralization. The positively charged proteins interact with negatively charged colloidal particles, reducing
repulsive forces and promoting aggregation. In contrast, polysaccharide-based coagulants such as chitosan and
plant-derived mucilage operate mainly through polymer bridging, where long-chain molecules link suspended
particles to form larger flocs that can be easily separated.
In addition to charge neutralization and polymer bridging, adsorption also plays a significant role in the
coagulation process, particularly for agro-waste-derived materials. These mechanisms often act simultaneously,
enhancing the overall efficiency of natural coagulants in removing turbidity, suspended solids, and organic
matter.
Several studies have reported that natural coagulants can achieve turbidity removal efficiencies of up to 90%,
along with substantial reductions in COD and BOD levels. For instance, Moringa oleifera and chitosan have
demonstrated effective pollutant removal under optimized conditions. However, the efficiency of these
coagulants is influenced by multiple factors, including pH, dosage, mixing conditions, and the characteristics of
the wastewater being treated.
Table 2 A: Protein-Based Natural Coagulants
S.NO
Coagulant
Component
Efficiency
Reference
1.
Moringa oleifera
Cationic proteins
High turbidity
removal
Rinaudo (2019); Abidin et
al. (2020)
2.
Cicer arietinum
Proteins
Moderate
Zaman et al. (2020)
3.
Vigna unguiculata
Proteins
Moderate
Mehta et al. (2021)
4.
Glycine max
Proteins
High
Das et al. (2023)
5.
Phaseolus vulgaris
Proteins
Moderate
[Zaman et al. (2020)
6.
Pisum sativum
Proteins
Moderate
Mehta et al. (2021)
7.
Dolichos lablab
Proteins
Moderate
Zhou et al. (2022)
8.
Tamarind seed kernel
Proteins
High
Mehta et al. (2021)
9.
Fenugreek seeds
Proteins
Moderate
Li et al. (2020)
10.
Groundnut cake
Proteins
Moderate
Wang et al. (2022)
Table 2B: Polysaccharide-Based Natural Coagulants
S.NO
Coagulant
Source
Component
Efficiency
Reference
1.
Chitosan
Shellfish
Polysaccharide
High COD removal
Garcia-Fayos et al. (2021);
Bratby (2016)
2.
Cactus
Plant
Mucilage
Moderate
Ong et al. (2020)
3.
Banana peel
Agro-waste
Cellulose
Moderate
Ahmad et al. (2018); Garcia-
Fayos et al. (2021)
4.
Potato starch
Potato
Starch
Moderate
Zaman et al. (2020)
5.
Corn starch
Maize
Starch
Moderate
Mehta et al. (2021)
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6.
Rice husk
Agro-waste
Cellulose
Moderate
Zhou et al. (2022)
7.
Okra mucilage
Abelmoschus
esculentus
Polysaccharide
High
Mehta et al. (2021)
8.
Aloe vera
Plant
Gel
polysaccharides
Moderate
Wang et al. (2022)
9.
Guar gum
Cyamopsis
tetragonoloba
Galactomannan
High
Li et al. (2020)
10.
Xanthan gum
Microbial
Polysaccharide
High
[Mehta et al. (2021)
They contain bioactive compounds such as proteins and polysaccharides that facilitate coagulation (Teh, et al.,
(2016), Saleem, et al., (2019), Zaman, et al., (2020), Mehta, et al., (2021)). These materials are renewable,
biodegradable, and environmentally friendly, making them suitable alternatives to chemical coagulants (Choy,
et al., (2017), Zhou, et al., (2022), Das, et al., (2023)).
Natural coagulants operate through multiple mechanisms including charge neutralization, polymer bridging, and
adsorption (Choy, et al., (2017), Ghernaout, (2020), Bhatia, et al., (2018)).
Table 3: Mechanisms vs Coagulant Type
S.NO
Mechanism
Description
Coagulant Example
1.
Charge Neutralization
Neutralizes particle charge
Moringa
2.
Polymer Bridging
Links particles
Chitosan
3.
Adsorption
Surface binding
Agro-waste
While natural coagulants demonstrate significant potential, their performance is not universally consistent across
different studies. Variations in raw material composition, extraction methods, and wastewater characteristics can
lead to fluctuations in treatment efficiency. For example, Moringa oleifera may achieve high turbidity removal
under optimal conditions, but its effectiveness in reducing COD and BOD can vary depending on initial pollutant
concentration and process parameters. Similarly, chitosan exhibits strong performance within a specific pH range
but may show reduced efficiency under highly alkaline conditions.
This variability underscores a key limitation in the application of natural coagulants: their effectiveness is highly
dependent on operational conditions. As a result, careful optimization and standardization are required to ensure
consistent performance, particularly in large-scale applications.
Comparison Between Natural and Chemical Coagulants
Several studies have quantitatively compared the performance of natural and chemical coagulants in wastewater
treatment. Chemical coagulants such as alum and ferric chloride can achieve turbidity removal efficiencies of
up to 95–99%, whereas natural coagulants typically achieve removal efficiencies ranging from 80–95%
depending on wastewater characteristics and dosage conditions (Chen, et al., (2021), Zhou, et al., (2022), Mehta,
et al., (2021)).
Natural coagulants such as Moringa oleifera and chitosan have demonstrated COD reduction efficiencies of 60–
85% and BOD reduction efficiencies of 50–80% (Rinaudo, (2019), Abidin, et al., (2020)). In contrast, chemical
coagulants often provide slightly higher removal rates but generate significantly larger volumes of non-
biodegradable sludge.
Additionally, sludge generated from natural coagulants is biodegradable and can be safely disposed of or reused
in agricultural applications, whereas chemical sludge may contain toxic metal residues requiring specialized
disposal methods. Economic analyses indicate that natural coagulants can reduce treatment costs by 20–50%
due to lower chemical usage and sludge handling costs (Chen, (2004), Ali, et al., (2021)).
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Table: 4 Comparison of Natural and Chemical Coagulants
S.NO
PARAMETER
NATURAL COAGULANTS
CHEMICAL COAGULANTS
1.
Source
Plant-based, biodegradable materials
Synthetic inorganic salts
2.
Cost
Low
High
3.
Efficiency
Moderate to high
High
4.
Sludge Production
Low, biodegradable
High, often hazardous
5.
Toxicity
Non-toxic
May cause toxicity
6.
Environmental Impact
Eco-friendly
Environmental concerns
Despite the generally reported performance ranges, significant variability exists in the efficiency of both natural
and chemical coagulants across different studies. This variation can be attributed to differences in wastewater
composition, pH, coagulant dosage, mixing conditions, and experimental scale. In the case of natural coagulants,
the absence of standardized extraction and preparation methods further contributes to inconsistencies in reported
results.
Moreover, most available studies are conducted under laboratory-scale conditions, which may not accurately
reflect real-world industrial scenarios. Factors such as fluctuating wastewater characteristics, operational
constraints, and economic considerations can influence performance at larger scales. Consequently, while natural
coagulants show considerable promise, their large-scale applicability requires further validation through pilot-
scale and industrial studies.
Operational Parameters Affecting Coagulation
The efficiency of coagulation depends on several factors such as pH, dosage, mixing conditions, and temperature.
Optimal pH for natural coagulants is typically between 6 and 8 (Zhang, et al., (2023), Bhatia, et al., (2018)).
Excess dosage can lead to particle restabilization, reducing efficiency (Zhang, et al., (2023)). Proper mixing
ensures uniform distribution and floc formation (Bhatia, et al., (2018), Vijayaraghavan, et al., (2020)).
Temperature influences reaction kinetics and can affect coagulation efficiency (Zhang, et al., (2023)). Careful
optimization of these parameters is essential for achieving consistent treatment performance (Zhang, et al.,
(2023), Bhatia, et al., (2018)).
Table 5 : Operational Parameters
S.NO
PARAMETER
OPTIMAL RANGE
EFFECT
1.
pH
6–8
Maximum efficiency
2.
Dosage
Varies
Overdose reduces efficiency
3.
Mixing Speed
Moderate
Ensures floc formation
4.
Temperature
Ambient
Affects kinetics
Multistage Treatment Approach
Multistage treatment systems integrate physical, chemical, and biological processes to achieve comprehensive
removal of pollutants from dairy wastewater (Gregory, 2018; Edzwald, 2017). Typically, primary treatment
involves coagulation–flocculation to remove suspended solids and colloidal matter, followed by biological
processes such as activated sludge systems or anaerobic digestion for the degradation of organic pollutants
(Ahmad et al., 2021). Tertiary treatment methods, including membrane filtration and advanced oxidation
processes, are subsequently employed to achieve higher levels of purification (Gregory, 2018; Edzwald, 2017).
The incorporation of natural coagulants into multistage systems has gained increasing attention due to their
potential to reduce chemical usage and sludge toxicity (Mehta et al., 2021). Several studies have demonstrated
that the use of natural coagulants in the primary stage can enhance overall treatment efficiency while improving
the sustainability of the process.
From a practical perspective, multistage systems offer greater flexibility in handling fluctuations in wastewater
composition and hydraulic load, which are common in dairy processing industries. However, the effectiveness
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of natural coagulants in such systems is highly dependent on operational conditions, including pH, dosage, and
mixing regimes.
Although promising results have been reported at laboratory scale, the transition to pilot-scale and full-scale
applications remains limited. Challenges such as process optimization, consistency in coagulant quality, and
integration with existing treatment infrastructure must be addressed to ensure successful implementation in
industrial settings (Mohd Salleh et al., 2019; Ali et al., 2021).
Economic and Sustainability Analysis
Natural coagulants reduce chemical procurement costs and sludge disposal expenses. Their use supports circular
economy principles by utilizing agricultural waste materials. Additionally, reduced environmental impact lowers
compliance costs associated with wastewater discharge regulations (Chen, (2004)). These benefits make natural
coagulants an economically viable and sustainable option for wastewater treatment.
Challenges and Future Perspectives
Despite the growing interest in natural coagulants, several challenges hinder their widespread application in
wastewater treatment. One of the primary limitations is the variability in raw material composition, which can
significantly influence coagulation performance (Choy et al., 2017; Zaman et al., 2020; Mehta et al., 2021).
Additionally, the lack of standardized extraction and preparation methods leads to inconsistencies in efficiency
across different studies.
Another important challenge is the relatively short shelf life and potential biodegradation of natural coagulants,
which may affect storage and long-term usability. In many cases, higher dosages are required compared to
chemical coagulants, which can impact process efficiency and operational costs (Zaman et al., 2020; Mehta et
al., 2021).
From an industrial perspective, scalability remains a critical concern. Most studies on natural coagulants have
been conducted under laboratory conditions, and there is limited data on pilot-scale or full-scale implementation.
Factors such as fluctuating wastewater characteristics, supply chain limitations for raw materials, and
compatibility with existing treatment systems must be carefully evaluated (Zhou et al., 2022; Mehta et al., 2021).
Future research should focus on developing standardized extraction techniques, improving the stability and
storage of natural coagulants, and optimizing process parameters for large-scale applications (Li et al., 2020;
Wang et al., 2022). Recent advancements in hybrid coagulant systems, combining natural and chemical
coagulants, have shown promising results in balancing efficiency and sustainability (Wang et al., 2022; Zhou et
al., 2022).
CONCLUSION
Natural coagulants have emerged as promising and sustainable alternatives to conventional chemical coagulants
for the treatment of dairy wastewater. Their biodegradability, low toxicity, and potential cost advantages make
them particularly attractive for environmentally responsible wastewater management. When integrated into
multistage treatment systems, natural coagulants can contribute to effective removal of suspended solids and
organic pollutants while reducing chemical dependency and sludge-related concerns.However, their
performance remains variable and strongly dependent on factors such as raw material composition, extraction
methods, and operational conditions. In comparison to chemical coagulants, which offer consistent and
predictable efficiency, natural coagulants require careful optimization to achieve reliable results. This highlights
the importance of standardization and process control for their practical application.
Furthermore, the transition from laboratory-scale studies to pilot- and industrial-scale implementation remains
limited. Challenges related to scalability, storage stability, and consistent supply of raw materials must be
addressed to enable widespread adoption. In this context, emerging approaches such as hybrid coagulant systems
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and nano-enhanced biopolymers offer promising pathways to improve performance and overcome existing
limitations.
Overall, natural coagulants represent a viable and sustainable solution for dairy wastewater treatment. Future
research should focus on large-scale validation, process optimization, and the development of standardized
protocols to ensure consistent and efficient performance. With continued advancements, these materials have the
potential to play a significant role in advancing sustainable wastewater treatment practices.
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