Antibacterial Activities of Daucus Carota Against Urinary Tract Pathogen Escherichia Coli Isolated from Urine Specimen Collected from The Students of Nnamdi Azikiwe University, Awka, Nigeria

Abstract: The rising prevalence of antimicrobial resistance has prompted an increased interest in natural remedies such as plant-based antimicrobials. Among these, Daucus carota (carrot) is recognized for its potential antibacterial and antifungal properties. This study investigated the antibacterial activity of Daucus carota extracts against Escherichia coli isolated from urine specimens. Furthermore, the study evaluated the antimicrobial susceptibility of these pathogens using carrot extracts that quantifies the major phytochemical constituents of the carrot rhizome. The antimicrobial susceptibility testing was carried out using the in-vitro method, wherein varying concentrations of carrot extracts (aqueous extracts of carrot) were applied to the test pathogen. Zones of inhibition were measured to assess the sensitivity of the pathogen Escherichia coli. The results revealed that carrot extracts exhibited significant antibacterial activity as (7mm, 5mm, 10mm, 1mm, 8mm) against tested pathogen. The phytochemical analysis revealed that carrot contains high levels of bioactive compounds, including flavonoids, alkaloids, tannins, saponins, resins, steroids, glycosides and phenolic compounds through phytochemical analysis. These compounds maybe responsible for the observed antimicrobial properties. The findings suggest that carrot extracts possess notable antimicrobial properties, particularly against bacteria responsible for urinary tract infections. This suggests that Daucus carota can be used as a natural alternative to ineffective synthetic antibiotics for treating infections caused by multidrug-resistant pathogens.

Medical Microbiology

1. Aggarwal, N., Leslie, S. (2024). Escherichia coli in the urinary tract. Encyclopedia of medicine,5(2), 111-5. [Google Scholar] [Crossref]

2. Akhtar, S., Rauf, A., Imran, M. (2019). Dietary and health promoting perspective of carrot polyphenol. Trends in food science technology, 66, 36-47. [Google Scholar] [Crossref]

3. Anger, J., Ackerman, L. (2019). Urinary tract infections. International journal of urology, 202, 282-289. [Google Scholar] [Crossref]

4. Aohajer, M., Musher, D. (2018). Clinical significance of Staphylococcus aureus. Scandinavian journal of infectious diseases, 45, 688-95. [Google Scholar] [Crossref]

5. Behzadi, P., Aghapour, R., Akbari, C. (2017). Effect of Salmonella species on urinary tract. Encyclopedia of medicine, 15, 3-4. [Google Scholar] [Crossref]

6. Carlsson, S., Wiklund, P., Engstrand, L. (2019). Bacterial growth in urine. British Journal of experimental pathology, 69, 759-70. [Google Scholar] [Crossref]

7. Carolina, A., Benita., Martinez, G. (2017). Pytochemical properties of Daucus carota L. post harvest biological technology, 127, 99-104. [Google Scholar] [Crossref]

8. Cortes, C., Esteve, M., Frigola, A. (2021). Changes in carotenoids. European food research technology, 221, 125-131. [Google Scholar] [Crossref]

9. Cornish, J., Lecamwahsam, J., Harrison, G. (2018). Host defense mechanisms in the urinary tract. Journal of clinical urology, 81, 83-6. [Google Scholar] [Crossref]

10. Elvira, l., Garcia, J. (2019). Nutritional importance of carotenoids and their antioxidant properties. Journal of clinical medicine, 8, 229. [Google Scholar] [Crossref]

11. Ipe, D., Horton, E., Utett, G. (2018). Strategies of microbes for persistence in urine. Front cell infection microbbiology, 26, 11-6. [Google Scholar] [Crossref]

12. Kochiashuili, D., Khuskivadze, A. (2022). Prevention and treatment of urinary tract infection. Journal of clinical urology, 190, 1981-1989. [Google Scholar] [Crossref]

13. Kucheria, R., Dasgupta, P., Sacks, S. (2021). New surfacing urinary tract infections. Postgraduate medical jounal, 952, 83-6. [Google Scholar] [Crossref]

14. Lala, V., Leslie, W., Minter, D. (2018). Disruption of the layer of mucus. British journal of molecular biology, 474, 49-53. [Google Scholar] [Crossref]

15. May, M., Schostak, M., Lebentrau, S. (2017). Recurrent urinary tract infections.Journal of medical microbiology, 15, 3-4. [Google Scholar] [Crossref]

16. Petrin, F., Hartman, L., Dubios, C. (2017). Morphological and biochemicalcharacteristics of Daucus carota L. Journal of plant pathology, 245, 737-747. [Google Scholar] [Crossref]

17. Phan, G., Remaut, H., Wang, T., Allen, W. (2017). Pathogenesis of urinary tract infection. Journal of molecular biology, 474, 49-53. [Google Scholar] [Crossref]

18. Subashchandrabose, S., Mobley, H. (2019). virulence and fitness determinants of uropathogenic Escherichia coli. Journal of microbiological spectrum, 3, 235-261. [Google Scholar] [Crossref]

19. Wang, L., Staner, G. (2022). Anthocyannins and their role in the prevention of cancer. Journal of medical health, 269, 281-290. [Google Scholar] [Crossref]

20. Wellenberg, L., Johanssan, E., Olson, M. (2017). Antimicrobial activity of Daucus carota L. Journal of clinical and food microbiology, 5, 60. [Google Scholar] [Crossref]

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