Biological Control of Meloidogyne incognita using Mycorrhizal Fungi in Central Sudan

There are many pests and diseases damaging both the quality and quantity of tomato production. Plant parasitic nematodes are one of them. They represent an important constraint on the delivery of global food security. Damage caused by plant-parasitic nematodes has been estimated at US$ 80 billion per year.   Effects of three concentrations of mycorrhizal fungi were studied for control of Root Knot Nematode. Different tomato accessions were screened for Root-Knot Nematode susceptibility. All the tested concentrations of mycorrhizal fungi on nematode infected plants were produce positive effects i.e. nematode numbers were highly decreased compare to control which also appear as improvement of the growth of infected plants at various degrees.  Among three tested concentrations, generally, middle concentration 5 ml of mycorrhiza is most effective followed by high one 6 ml of mycorrhizea and the lowest effect produced by lower concentration 4 ml of mycorrhizea. Mycorrhizea fungi appear as most effective control measures of root-knot nematode in tomato.

Meloidogyne, Mycorrhizal, Mycorrhizal Central Sudan

1. Salazar, M.M. and Schroeder Using A Galling Index To Explore Root-Knot Nematode Biology And Epidemiology. The Plant Health Instructor. 2018. [Google Scholar] [Crossref]

2. Abd-Elgawad , M.M.M. Optimizing Safe Approaches to Manage Plant-Parasitic Nematodes. Plants (Basel). 2021 Sep 14;10(9):1911. doi: 10.3390/plants10091911. Špela Mechora (Ed). 2021. [Google Scholar] [Crossref]

3. Adam, A. B., Musa Yahaya Abubakar and Aliyu Mohammed . Investigating Natural Products as Pesticides alternatives : Study on Efficacy and Safety. (Conference Paper). BİLSEL INTERNATIONAL HARPUT SCIENTIFIC RESEARCHES CONGRESS, 09-10 November, 2024, ELAZIĞ/TÜRKİYE. 2024. [Google Scholar] [Crossref]

4. Anjos, E.C.T; Cavaclcante, U.M.T; Santos, V.F.S. and Maia, L.C.Prodcao de mudas de maracujazeiro-doc micorrizadas em solo desinfestado e adubado com fosforo. Pesquisa Agropecuaria Brasileir, . 2005: 40: 345-351. [Google Scholar] [Crossref]

5. Fabien, Baldacci-Cresp 1, Mickaël Maucourt 2, Catherine Deborde 3, Olivier Pierre 4, Annick Moing 3, Renaud Brouquisse 4, Bruno Favery 4, Pierre Frendo. Maturation of nematode-induced galls in Medicago truncatula is related to water status and primary metabolism modifications. Plant Science. V. 232. 2015. 77-85. [Google Scholar] [Crossref]

6. Candido, V., Gabriele Campanelli, Trifone D’Addabbo, Donato Castronuovo, Marek Renco, and Ippolito Camele (2013). Growth and Yield Promoting Effect of Artificial Mycorrhization Combined with Different Fertilizer Rates on Field-Grown Tomato. Italian Journal of Agronomy [Google Scholar] [Crossref]

7. Cofcewicz, E. T., Medeiros, C. A. B. and Carneiro, R. M. D. G. and Pierobom, C. R. (2001). Interaction of fungus micorrizicos arbuscular Glomas [Google Scholar] [Crossref]

8. Cuvaca, J.,Isabel Abrantes, Carla Maleita and Ivânia Esteves (2026). Occurrence and Management of Plant-Parasitic Nematodes in Mozambique: A Review. Crops 2026, 6(1), 6; https://doi.org/10.3390/crops6010006. [Google Scholar] [Crossref]

9. Elling, A. A. (2013). Major emerging problems with minor Meloidogyne Species. Phytopathology,103:1092-1102. [Google Scholar] [Crossref]

10. FAO. (2010). Plant genetic resource for food and agriculture. Rome, Italy, Food and Agriculture Organization of the United Nations. [Google Scholar] [Crossref]

11. FAO. (2012). Food and Agricultural Organization statistics book on national crop production. Rome, Italy, Food and Agriculture Organization of the United Nations. [Google Scholar] [Crossref]

12. Giovanetti, M. and B. Mosse (1980). An evaluation of techniques for measuring vesicular mycorrhizal infection in roots. New Phytologist 97:447-453. [Google Scholar] [Crossref]

13. Giovannucci, E. (1999). Tomatoes, Tomato-Based Products, Lycopene, and Cancer: Review of the Epidemiologic Literature. Journal of the National Cancer Institute, Volume 91, Issue 4, 17, Pages 317–331, https://doi.org/10.1093/jnci/91.4.317. [Google Scholar] [Crossref]

14. Hodge A. (2000). Microbial ecology of the arbuscular mycorrhiza. FEMS Microbiol. Ecol. 32, 91–96. 10.1111/j.1574-6941.2000.tb00702.x [DOI] [PubMed] [Google Scholar. [Google Scholar] [Crossref]

15. Howland, D.A. and Marisol Quintanilla (2023). Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review. J Nematol. 2023 Apr 14;55(1):20230007. doi: 10.2478/jofnem-2023-0007. [Google Scholar] [Crossref]

16. Huang, B., Li, J., Wang, Q., Guo, M., Yan, D., Fang, W., Ren, Z., Wang, Q., Ouyang, C., Li, Y., Cao, A., (2018). Effect of soil fumigants on degradation of Abamectin and their combination synergistic effect to root-knot nematode.PLoSOne13,e0188245.https ://doi.org/10.1371/journal.pone.0188245. [Google Scholar] [Crossref]

17. Hussey, R. S. and Barker, K. R., (1973). Comparison of methods for collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 7, 1025-1028. [Google Scholar] [Crossref]

18. Jaouannet, M. and Rosso, M. (2013). Effectors of root sedentary nematodes target diverse plant cell compartments to manipulate plant functions and promote infection. Plant Signal Behav. 2013 Jul 1;8(9):e25507. doi: 10.4161/psb.25507. [Google Scholar] [Crossref]

19. Jones D. L., Hodge A., Kuzyakov Y. (2004). Plant and mycorrhizal regulation of rhizodeposition.New Phytol. 163, 459–480. 10.1111/j.1469-8137.2004.01130.x [DOI] [PubMed] Google Scholar. [Google Scholar] [Crossref]

20. Kim, D. I., Park, J. D., Kim, S. G., KuK, H. Jang, M. J. and S. S., (2005). Screening off some crude plant extracts for their acaricidal and insecticidal afficacies. J. Asain Pacific Entomol. 8, 93-100. [Google Scholar] [Crossref]

21. Kim, D.G., Seung-Han Kim and Joong-Hwan Lee (2001). Observation of Root-knot Nematodes in the Root Gall Formed on Oriental Melon. Plant Pathol. J. 21(1): 73-76 (2005). [Google Scholar] [Crossref]

22. Kumar, A.D.D. and Donthi, R.N. (2024). Adverse Effects of Pesticides: Regulatory Failures, Impacts on Public Health and Environmental Wellbeing. In book: Insecticides in Pest Control - Impact, Challenges and Strategies [Working Title]. License CC BY 3.0. [Google Scholar] [Crossref]

23. Matlala, F. L., Hendrika Fourie, Wiam Haddad, Dirk De Waele and Mieke Stefanie Daneel (2025). Prevalence of plant-parasitic nematodes in nethouse tomato production in Limpopo Province, South Africa, and relationships with physico-chemical soil properties. Journal of Plant Diseases and Protection, Volume 132, article number 140, [Google Scholar] [Crossref]

24. Mfarrej M.F. B. and Rara F.M. (2019). Competitive, Sustainable Natural Pesticides. Acta Ecologica Sinica, Volume 39, Issue 2, April 2019, Pages 145-151. [Google Scholar] [Crossref]

25. Moens, M; Roland, N. P. and James, L S. (2009). "Meloidogyne Species: a diverse group of novel and important plant parasites." In: Root-knot Nematodes, ed. Roland, N P; Maurice, M, and James, L. S. Wallingford, UK: CABI Publishing. Pp 1-17. [Google Scholar] [Crossref]

26. Nerva L., Raffaella Maria Balestrini and Walter Chitarra. From Plant Nursery to Field: Persistence of Mycorrhizal Symbiosis Balancing Effects on Growth-Defence Tradeoffs Mediated by Rootstock. Agronomy. 2023: 13(1):229 [Google Scholar] [Crossref]

27. Nicol, J. M; Turner, S. J. D; Coyne, D. L; Nijs, L. D; Hockland, S. and Maafi, Z. T. (2011). Current nematode threats to world agriculture. In:J. Jones, G. Gheysen, and C. Fenoll, (Eds) Genomics and molecular genetics of plant-nematode interactions. New York: Springer. Pp. 21-43. [Google Scholar] [Crossref]

28. Nicol, J.M., Turner, S.J., Coyne, D.L., Den Nijs, L., Hockland, S. and Maafi, Z.T. (2011). Current nematode threats to world agriculture. In: Jones, J.T., Gheysen, G. & Fenoll, C. (Eds). Genomics and molecular genetics of plant-nematode interactions. Heidelberg, Germany, Springer, pp. 21-44. [Google Scholar] [Crossref]

29. Osman, A. A and D. V. Viglierchio. (1998). Efficacy of biologically active agents as nontraditional nematicides for Meloidoyne javanica. Reveue Nematol. 11: 93-98. [Google Scholar] [Crossref]

30. Palomares-Rius, J. Carolina Escobar, Javier Cabrera Alessio Vovlas and Pablo Castillo (2017). Anatomical Alterations in Plant Tissues Induced by Plant-Parasitic Nematodes. Front Plant Sci. 2017 Nov 16;8:1987. doi: 10.3389/fpls.2017.01987. [Google Scholar] [Crossref]

31. Prsanthi, S. Jalander V. and M. Mamatha (2016). The Vesicular Arbuscular Mycorrhizal Associations with Two Medicinal Plant Species in Telangana University Campus, Nizamabad (T.S.), India. International Journal of Current Research in Biosciences and Plant Biology ISSN: 2349-8080 (Online), 3(1): 62-65. [Google Scholar] [Crossref]

32. Rady, G. H. H.; Abdelnabby, H. M.; Gohar, I. M. A.; Mohamed, Gh. R. Y.; and Sabra, T. F. Y. (2021). Evaluation of Some Root-knot Nematode Management Strategies in Sugar beet Fields at West Nubaryia District. 5th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, 8 April 2021,Egypt (Conference Online). [Google Scholar] [Crossref]

33. Schouteden, N, Dirk De Waele , Bart Panis and Christine M. Vos . (2015). Arbuscular Mycorrhizal Fungi for the Biocontrol of Plant-Parasitic Nematodes: A Review of the Mechanisms Involved. Front Microbiol. 17;6:1280. doi: 10.3389/fmicb.2015.01280. PMCID: PMC4646980 PMID: 26635750. [Google Scholar] [Crossref]

34. Seid A., Fininsa, C., Mengistu, T.M., Decraemer W. and Wesemael W.M. (2015). Tomato (Solanum lycopersicum) and root-knot nematodes (Meloidogyne spp.) – a century-old battle. Nematology 17(9):1-15. [Google Scholar] [Crossref]

35. Siddiqui. Y, Asgar Ali and Yuvarani Naidu (2014). Histopathological changes induced by Meloidogyne incognita in some ornamental plants. Crop Protection, v. 65. p.216-220, 2014. [Google Scholar] [Crossref]

36. Talavera, M., Itou, K. and Mizukubo. T. (2001). Reduction of nematode damage by root colonization with arbuscular mycorrhiza (Glomus spp) in tomato-Meloidogyne incognita (Tylenchida: Meloidogynidae) and carrot-Pratylenchus penetrans (Tylenchidae) pathosystems. Applied Entomology and Zoology, 36, 387-392. [Google Scholar] [Crossref]

37. Taylor, A. L, Sasser, J. N. (1978). Biology, Identification and control of Root-Knot Nematodes (Meloidogyne species). North Carolina State University, U. S. Agency for International Development, Raleigh. North Carolina. Pp 111. [Google Scholar] [Crossref]

38. Taylor, A. L. (1967). An introduction to research on plant nematology. FAO. Guide study and control of plant parasitic nematodes. Pp 1-4. [Google Scholar] [Crossref]

39. Viaene, N. M., Abawi, G. S. Management of Meloidogyne hpla on lettuce in organic soil with Sudangrass as a cover crop. Plant Dis. 1998. : 945-952. [Google Scholar] [Crossref]

40. Villaverde, J.J., Sevilla-Moran, B., Lopez-Goti, C., Alonso-Prados, J.L., Sandín-Espa~na, PTrends in analysis of pesticide residues to fulfil the European Regulation (EC) No. 1107/2009. Trends Anal. Chem. 2016. : 80, 568–580. https://doi.org/10.3390/ molecules2305099. [Google Scholar] [Crossref]

41. Volume 8, Issue 3, 2013, e22. https://doi.org/10.4081/ija.2013.e22. [Google Scholar] [Crossref]

42. Volume: 18, , Article Type: Lab Exercise. DOI:10.1094/PHI-I-2018-1218-01. [Google Scholar] [Crossref]

43. Williams, S.D. Michael J. Boehm, and Horacio Lopez-Nicora. Nematode Diseases of Plants. Ohioline. Ohio State University Extension. https://ohioline.osu.edu/.2017. [Google Scholar] [Crossref]

44. Yan, D., Reza Ghaderi, Jizheng He, Aocheng Cao and Qiuxia Wang Recent Advances in Nematicides and Their Modes of Action. Agriculture 2026: 16(1), 21. https://doi.org/10.3390/agriculture16010021. [Google Scholar] [Crossref]

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