Invitro Antimicrobial Properties of Gold Nanaoparticles Biosynthesized by Medicinal Plant Extracts: Croton Megalocarpus

Abstract: Nanoparticles are small particles with distinctive characteristics of chemical, biological and physical properties as compared to the bulk matter. Great interest to explore the potential and application of this nanoparticles have gain momentum in the field of science. This research in particular was designed to explore alternative way of producing these nanoparticles. Green synthesis used in this research was prefer as the materials are readily available, the method is ecofriendly and potentially efficient. The study utilized croton megalocarpus leaves extract as reducing and capping agent of gold solution (1 mM gold (III) chloride trihydrate).Formation of AuNPs was done using Uv vis spectrophotometer while characterization was done using HRTEM,the active biomecules present in the plant extract was screened using FTIR. From the results the stirring time was six minutes and the color of the solution turned from right yellow to dark brown. The uv vis spectrophotometer surface plasmon resonance (SPR) was λ_max 545 nm with the intensity of 1.0 after six minutes. The HRTEM results indicated that the resultant nanoparticle were mostly spherical with non-uniform surface with an average diameter of 26.5±1.2nm. The FTIR indicated O-H stretching at 3273.26 cm^-1+ absorbance peak which corresponds to alcohol and phenol, carbonyl (C=O) groups stretching at an absorbance peak of 1637.59 cm^-1 corresponding to carbonyl-containing organic species. The nanoparticles produced showed great antimicrobial properties especially on gram negative bacteria, with E.coli having highest inhibition of 15 ±0.41nm  the least affected was B.sabtilis a gram positive bacteria with 8.5 ± 0.73 and E.feacalis which was resistant to AuNPs inhibition. Synergistic antimicrobial effect was observed on E.feacalis where coating an inhibition of 16.5nm as compared to drug alone which had an inhibition of 14.5nm. These findings suggest that AuNPs synthesized from Croton megalocarpus could be valuable in the development of nanotechnology-based antimicrobial drugs.

MEDICAL HEALTH

1. Čiginskienė, A. et al. (2019) “Ventilator-associated pneumonia due to drug-resistant Acinetobacter baumannii: Risk factors and mortality relation with resistance profiles, and independent predictors of in-hospital mortality,” Medicina, 55(2), p. 49. Available at: https://doi.org/10.3390/medicina55020049 [Google Scholar] [Crossref]

2. Silva, Ana C., et al. “Determinants of Antimicrobial Resistance among the Different European Countries: More than Human and Animal Antimicrobial Consumption.” Antibiotics, vol. 10, no. 7, 2021, p. 834., https://doi.org/10.3390/antibiotics10070834. [Google Scholar] [Crossref]

3. “The future of antibiotics – has resistance switched out the light at the end of the tunnel?” (2001) Magic Bullets, Lost Horizons, pp. 243–264. Available at: https://doi.org/10.1201/b12641-11. [Google Scholar] [Crossref]

4. Ayukekbong, J., Ntemgwa, M., &Atabe, A. (2017). The threat of antimicrobial resistance in developing countries: causes and control strategies. Antimicrobial Resistance & Infection Control, 6(1). doi: 10.1186/s13756-017-0208 [Google Scholar] [Crossref]

5. Heifets, Leonid, and Gerard Cangelosi. “Drug Resistance Assays for Mycobacterium Tuberculosis.” Antimicrobial Drug Resistance, 2009, pp. 1161–1170., https://doi.org/10.1007/978-1-60327-595-8_35. [Google Scholar] [Crossref]

6. Michael, C., Dominey-Howes, D., &Labbate, M. (2014). The Antimicrobial Resistance Crisis: Causes, Consequences, and Management. Frontiers In Public Health, 2. doi: 10.3389/fpubh.2014.00145 [Google Scholar] [Crossref]

7. Mohammadi, B. and Salouti, M. (2014) “Extracellular bioynthesis of silver nanoparticles by penicillium chrysogenum and penicillium expansum,” Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 45(6), pp. 844–847. Available at: https://doi.org/10.1080/15533174.2013.862640. [Google Scholar] [Crossref]

8. Drug resistance updates meetings-courses-workshops” (2003) Drug Resistance Updates, 6(4), pp. 225–230. Available at: https://doi.org/10.1016/s1368-7646(03)00066-9. [Google Scholar] [Crossref]

9. De Jong, W. H., &Borm, P. J. (2008). Drug delivery and nanoparticles: Applications and hazards. International Journal of Nanomedicine, 3(2), 133–149. [Google Scholar] [Crossref]

10. Munir, M.M., Ogi, T. and Okuyama, K. (2018) “Nanoparticle synthesis, dispersion, and functionalization for Industrial Application,” Nanoparticle Technology Handbook, pp. 675–681. Available at: https://doi.org/10.1016/b978-0-444-64110-6.00059-7. [Google Scholar] [Crossref]

11. Dakrong P, Takuro N, Michael B (2011) The forthcoming applications of gold nanoparticles in drug and gene delivery systems. Journal of Controlled Release 149(1): 65-71. [Google Scholar] [Crossref]

12. springer (no date) “Dynamic properties of nanoparticles,” SpringerReference [Preprint]. Available at: https://doi.org/10.1007/springerreference_583. [Google Scholar] [Crossref]

13. Hirakawa, K. (2015) “Fundamentals of medicinal application of titanium dioxide nanoparticles,” Nanoparticles Technology [Preprint]. Available at: https://doi.org/10.5772/61302.(13 [Google Scholar] [Crossref]

14. Li, J., Wu, Q. and Wu, J. (2015) “Synthesis of nanoparticles via solvothermal and hydrothermal methods,” Handbook of Nanoparticles, pp. 1–28. Available at: https://doi.org/10.1007/978-3-319-13188-7_17-1. [Google Scholar] [Crossref]

15. Rai A, Prabhune A, Perry CC (2010) Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings. J Mater Chem 20: 6789-6798 [Google Scholar] [Crossref]

16. Saranya S. Biosynthesis of gold nanoparticles (AuNPs) from C. orchioidesand study their antimicrobial efficacy. Int. J. Phytopharm. 2015, 5, 58–64. [Google Scholar] [Crossref]

17. Sehgal, N., Soni, K., Gupta, N., &Kohli, K. (2016). Microorganism Assisted Synthesis of Gold Nanoparticles: A Review. Asian Journal Of Biomedical And Pharmaceutical Sciences, 22. Retrieved from http://www.alliedacademies.org/articles/microorganism-assisted-synthesis-of-gold-nanoparticles-a-review.pdf [Google Scholar] [Crossref]

18. Modeling the size dependency of the stability of metal nanoparticles” (no date). Available at: https://doi.org/10.1021/acs.jpcc.9b06952.s001. [Google Scholar] [Crossref]

19. Mohammadi, B. and Salouti, M. (2014) “Extracellular bioynthesis of silver nanoparticles by penicillium chrysogenum and penicillium expansum,” Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 45(6), pp. 844–847. Available at: https://doi.org/10.1080/15533174.2013.862640. [Google Scholar] [Crossref]

20. Foliatini, F., Yulizar, Y., &Hafizah, M. (2015). Microwave-Assisted Synthesis of Alginate-Stabilized Gold Nanoparticles. Makara Journal Of Science, 18(4). doi: 10.7454/mss.v18i4.4281. [Google Scholar] [Crossref]

21. Aboyewa, J.A. et al. (2021) “Green synthesis of metallic nanoparticles using some selected medicinal plants from southern Africa and their biological applications,” Plants, 10(9), p. 1929. Available at: https://doi.org/10.3390/plants10091929. [Google Scholar] [Crossref]

22. Kuppusamy, P., Yusoff, M., Maniam, G., &Govindan, N. (2016). Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – An updated report. Saudi Pharmaceutical Journal, 24(4), 473-484. doi: 10.1016/j.jsps.2014.11.013 [Google Scholar] [Crossref]

23. Darweesh, M. et al. (2021) “Unique, inexpensive, and abundantly available in nature adsorbent of synthesized silver nanoparticles (agnps) /banana leave powder (BLP) composite,” SSRN Electronic Journal [Preprint]. Available at: https://doi.org/10.2139/ssrn.3981529. [Google Scholar] [Crossref]

24. M., N. et al. (2018) “Biosynthesis, characterization, and evaluation of bioactivities of leaf extract-mediated biocompatible gold nanoparticles from Alternanthera Bettzickiana,” Biotechnology Reports, 19. Available at: https://doi.org/10.1016/j.btre.2018.e00268. [Google Scholar] [Crossref]

25. Zawrah MF, Abd El-Moez SI (2011). Antimicrobial Activities of Gold Nanoparticles against Major Foodborne Pathogens. Life Science Journal 8(4); 37-44 [Google Scholar] [Crossref]

26. Zignol, Matteo, et al. “Surveillance of Anti-Tuberculosis Drug Resistance in the World: An Updated Analysis, 2007–2010.” Bulletin of the World Health Organization, vol. 90, no. 2, 2011, https://doi.org/10.2471/blt.11.092585. [Google Scholar] [Crossref]

27. Zha, J. et al. (2017) “Green synthesis and characterization of monodisperse gold nanoparticles using ginkgo biloba leaf extract,” Optik, 144, pp. 511–521. Available at: https://doi.org/10.1016/j.ijleo.2017.06.088. [Google Scholar] [Crossref]

28. Abimbola Akinsiku, A., Oluseyi Ajanaku, K. and Olugbenga Dare, E. (2019) “Green synthesis of pseudo-cubic ag/ni bimetallic nanoparticles using Senna occidentalis leaf extract,” Journal of Physics: Conference Series, 1299(1), p. 012133. Available at: https://doi.org/10.1088/1742-6596/1299/1/012133. [Google Scholar] [Crossref]

29. Maroyi, A. (2017) “Croton megalocarpus hutch. in tropical Africa: Phytochemistry, pharmacology and medicinal potential,” Research Journal of Medicinal Plants, 11(4), pp. 124–133. Available at: https://doi.org/10.3923/rjmp.2017.124.133. [Google Scholar] [Crossref]

30. Ozdal, M. and Gurkok, S. (2022a) ‘A recent advances in nanoparticles as antibacterial agent’, ADMET and DMPK [Preprint]. doi:10.5599/admet.1172. [Google Scholar] [Crossref]

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