Development of a Single-point Optical Scanning System for Teaching Transmission Electron Microscopy Principles
Authors
Romero, Oscar Jr. O
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Bocayong, Apple Jhen P
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Demecillo, Debie Jannen R
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Guangco, Isaiah Gelmar C
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Humdos, Dana T
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Oguis, Uhxia M
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Clint Titus P
Grade 12-STEM, Mindanao State University-Maigo College of Education Science and Technology, Philippines (PH)
Article Information
DOI: 10.51583/IJLTEMAS.2026.150500148
Subject Category: STEM
Volume/Issue: 15/5 | Page No: 1871-1879
Publication Timeline
Submitted: 2026-06-09
Published: 2026-06-09
Abstract
Transmission Electron Microscopy (TEM) is an important scientific imaging technique; however, students often find its underlying principles difficult to understand because of the abstract nature of electron optics and the limited availability of microscopy equipment in educational settings. This study developed and evaluated a low-cost Single-Point Optical Scanning Instructional System designed to demonstrate the fundamental principles of TEM through hands-on and visualization-based learning.
The prototype utilized a laser module, optical sensors, stepper motors, a turntable mechanism, an Arduino Uno microcontroller, and image reconstruction software to simulate scanning, signal detection, and image formation processes. A mixed-methods project-based research design was employed involving ten Grade 12 STEM students. Participants completed pre-test and post-test assessments to measure conceptual understanding before and after exposure to the instructional demonstration. Results showed an increase in mean scores from 8.20 to 12.40, representing a 51.22% improvement.
A paired-samples t-test indicated that the increase was statistically significant, t(9) = 8.20, p < 0.001. The findings suggest that the developed instructional system effectively improves students’ understanding of TEM principles while providing an affordable alternative to expensive microscopy equipment. The study highlights the potential of low-cost, interactive STEM instructional tools for enhancing science education in resource-limited learning environments.
Keywords
Development, Single-point Optical, Scanning System
Downloads
References
1. Adachi, Y., Yamamoto, N., & Sannomiya, T. (2023). Focused light introduction into transmission electron microscope via parabolic mirror.. Ultramicroscopy, 251, 113759 . https://doi.org/10.1016/j.ultramic.2023.113759 [Google Scholar] [Crossref]
2. Adi, N. H., & Azra, F. (2023). Students’ difficulties in learning atomic structure. Journal of Education and Learning (EduLearn), 17(2), 267–274. https://doi.org/10.11591/edulearn.v17i2.22475 [Google Scholar] [Crossref]
3. Alcorn, F. M., Jain, P. K., & van der Veen, R. M. (2023). Time-resolved transmission electron microscopy for nanoscale chemical dynamics. Nature Reviews Chemistry, 7(4), 256–272. https://doi.org/10.1038/s41570-023-00469-y [Google Scholar] [Crossref]
4. Aycan, S., Altun, E., Yerdelen, S., & Göksu, V. (2019). Students’ mental models of the atom and their difficulties in learning abstract atomic concepts. Journal of Baltic Science Education, 18(1), 9–23. https://doi.org/10.33225/jbse/19.18.09 [Google Scholar] [Crossref]
5. Benjin, X., Liu, J., & others. (2020). Developments, applications, and prospects of cryo- electron microscopy (cryo-EM). Protein Science, 29(1), 39–52. https://doi.org/10.1002/pro.3805 [Google Scholar] [Crossref]
6. Cheng, Y. (2018). Single-particle cryo-EM—How did it get here and where will it go. Science, 361(6405), 876–880. https://doi.org/10.1126/science.aat4346 [Google Scholar] [Crossref]
7. Dablio, A. R., Lagmay, M., Margarito, M., de Yro, P. A., & others. (2024). Philippines’ success in interlaboratory comparisons of nanoparticle geometric size measurements. Measurement Sensors, 38, Article 101527. https://doi.org/10.1016/j.measen.2024.101527 [Google Scholar] [Crossref]
8. Da Cunha, M. B., dos Santos, F. M. T., & Giordan, M. (2023). Students’ use of quantum and Bohr models of the atom: A representational versus conceptual understanding. Research in Science Education, 53, 151–170. https://doi.org/10.1007/s11165-021- 10023-1 [Google Scholar] [Crossref]
9. de Broglie, L. (1924). Recherches sur la théorie des quanta [Research on the quantum theory] (Doctoral dissertation, University of Paris). Annales de Physique, 10(3), 22–128. [Google Scholar] [Crossref]
10. Dongre, A., Joshi, A., & Kapadia, M. (2012). Enhancing Conceptual Understanding through Hands-on Practical Tools in Science Education. arXiv. https://arxiv.org/abs/1205.1141 [Google Scholar] [Crossref]
11. Gabor, D. (1946). Theory of electron optics: A new approach to electron microscopy. Panganiban, R. E. (2020). The effectiveness of indigenous and low-cost teacher-made science instructional materials in selected third year students of the Balayan National High School. Instabright International Journal of Multidisciplinary Research, 2(1), 49–52. Retrieved from https://instabright.online/index.php/journal/article/view/8 [Google Scholar] [Crossref]
12. Freed, N., et al. "An Interactive Online Electron Microscopy Platform Integrating Classroom Lectures and Lab Practice." Microscopy Today, vol. 28, 2020, pp. 46 - 51. https://doi.org/10.1017/s1551929520000656. [Google Scholar] [Crossref]
13. Galaz-Montoya, J. G. (2024). The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography. Frontiers in Molecular Biosciences, 111390858. https://doi.org/10.3389/fmolb.2024.1390858 [Google Scholar] [Crossref]
14. Haider, M., Uhlemann, S., Schwan, E., Rose, H., Kabius, B., & Urban, K. (1998). Electron microscopy image enhanced. Nature, 392(6678), 768–769. https://doi.org/10.1038/33823 [Google Scholar] [Crossref]
15. a. https://doi.org/10.1119/1.18165 [Google Scholar] [Crossref]
16. Koguchi, M., Tsunekawa, Y., Tsunoyama, K., & Banerjee, I. A. (2015). Electron tomography: A three-dimensional analytic tool for hard and soft materials research. Advanced Materials, 27(38), 5638–5663. https://doi.org/10.1002/adma.201501015 [Google Scholar] [Crossref]
17. Kumar, A., Sharma, P., & Singh, R. (2022). The Role of Virtual Microscopy in Science Education: Benefits and Challenges. Computers & Education, 180, 104458. https://www.sciencedirect.com/science/article/pii/S0377123722000181 [Google Scholar] [Crossref]
18. Lam, Matilynn, et al. "An Introduction to Scanning Electron Microscopy and Science Communication Skills for Undergraduate Chemistry Students." Journal of Chemical Education, 2023. https://doi.org/10.1021/acs.jchemed.3c00076. [Google Scholar] [Crossref]
19. Lim, Sin Ting, et al. "An Educational CT Scanner Prototype Using Optical Scanning." 2024 Multimedia University Engineering Conference (MECON), 2024, pp. 1-5. https://doi.org/10.1109/mecon62796.2024.10776174. [Google Scholar] [Crossref]
20. Liu, X., & Lesniak, K. M. (2016). Progression in students’ understanding of the matter concept from elementary to high school. Journal of Research in Science Teaching, 53(5), 683– 708. https://doi.org/10.1002/tea.21312 [Google Scholar] [Crossref]
21. Liu, Zhongwei. "Design and Implementation of an Integrated Teaching Approach for Transmission Electron Microscopy Laboratory Education." International Journal of Multidisciplinary Research and Growth Evaluation, 2025. https://doi.org/10.54660/.ijmrge.2025.6.6.1103-1106. [Google Scholar] [Crossref]
22. Low, Darren Yi Sern, et al. "Improving Student Motivation and Learning in Chemical Engineering Education: A Case of Scanning Electron Microscopy with Virtual 3D Technology." Education for Chemical Engineers, 2025. https://doi.org/10.1016/j.ece.2025.100498. [Google Scholar] [Crossref]
23. Magnani, L., Rossi, M., & Bianchi, F. (2025). Accessibility Challenges in Microscopy Education: A Review of Low-Cost Alternatives. Journal of Microscopy Education, 12(1), 45–59. https://pubmed.ncbi.nlm.nih.gov/39611369/ [Google Scholar] [Crossref]
24. Nguyen, K. X., Yuan, R., Brown, H. G., Chen, M., Sunku, S. S., & Ercius, P. (2024). Achieving sub-0.5-angstrom–resolution ptychography in an uncorrected scanning transmission electron microscope. Science, 384(6694), 522–527. https://doi.org/10.1126/science.adl2029 [Google Scholar] [Crossref]
25. Padilla, Hurtado, and Juan Pablo. "Electron microscopes as educational tools: The use of a Scanning Electron Microscope to develop 3D models for educational programs." Microscopy and Microanalysis, vol. 26, 2020, pp. 65 - 66. https://doi.org/10.1017/s1431927620000562. [Google Scholar] [Crossref]
26. Pennycook, S. J., Lupini, A. R., Varela, M., & Hetherington, C. J. D. (2003). Sub-Ångstrom resolution through aberration-corrected STEM. Microscopy and Microanalysis, 9(S02), 926–927. https://doi.org/10.1017/S1431927603444632 [Google Scholar] [Crossref]
27. Prameela, Suhas Eswarappa, et al. "Looking at education through the microscope." Nature Reviews. Materials, vol. 5, 2020, pp. 865 - 867. https://doi.org/10.1038/s41578-020-00246-z. [Google Scholar] [Crossref]
28. Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, 197(1051), 454–467. https://doi.org/10.1098/rspa.1949.0005 [Google Scholar] [Crossref]
29. Ruska, E. (1987). The development of the electron microscope and of electron microscopy. Reviews of Modern Physics, 59(3), 627–638. https://doi.org/10.1103/RevModPhys.59.627 [Google Scholar] [Crossref]
30. Ullah, N., Qazi, R. A., Ullah, S., & Khan, S. (2022). Application and importance of scanning and transmission electron microscopes in science and technology. Contributions, Section of Natural, Mathematical and Biotechnical Sciences, 43(1–2), 27–37. https://doi.org/10.20903/masa/nmbsci.2022.43.13 [Google Scholar] [Crossref]
31. Waheed, Malaika, et al. "Design and development of a portable compound microscope for interactive bioscience learning." , vol. 13024, 2024, pp. 130240Q - 130240Q-6. https://doi.org/10.1117/12.3022127. [Google Scholar] [Crossref]
32. Wolf, Vanessa, et al. "Utilization of Remote Access Electron Microscopes to Enhance Technology Education and Foster STEM Interest in Preteen Students." Research in Science Education, vol. 52, 2020, pp. 617 - 634. https://doi.org/10.1007/s11165-020-09964-4. [Google Scholar] [Crossref]
33. Zhang, Chengyi, et al. "Integrating Laser-scanning Technology into a Construction Engineering and Management Curriculum." 2021 ASEE Virtual Annual Conference Content Access Proceedings, 2024. https://doi.org/10.18260/1-2--37361. [Google Scholar] [Crossref]
34. ZEISS Microscopy Education. (2024). Teaching Microscopy in Resource-Limited Settings. Carl Zeiss Microscopy. https://www.zeiss.com/microscopy/en/applications/education-teaching.html [Google Scholar] [Crossref]
Metrics
Views & Downloads
Similar Articles
- Block-Based Programming for Education: A Comprehensive Analysis of Visual Programming Environments in K-12 Learning
- Management of Academic Libraries and Client Satisfaction Towards Digital Utilization: Basis for Monitoring Library Operations in SOCCSKSARGEN Region.
- Revenue Leakages in TPA Insurance Claims and Corporate Claims: An Institutional Overview of Aster Prime Hospital, Hyderabad
- Technology and Innovation in Hospitality and Tourism: A Management Perspective
- Geospatial Distribution of Tarok Sacred Grove of Langtang North and Langtang South Local Government Areas