Comparative Studies on Lipase Production and Optimization using Bacillus Subtilis and Pseudomonas Aeruginosa
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The presents study deals with screening of lipase producers from the lipase rich environment like petrol bank, Auto mobile shops from soil sample. The micro organisms have the ability to produce the lot amount of lipase. The sample was collected from (petrol bank, Auto mobile shops). Tributyrin agar medium was prepared 14 strains were isolated from that 2 strains able to produce lipase. The bacterial strain confirmed by biochemical method. The isolated bacterial strain are Bacillus sp. and Pseudomonas sp. The bacterial strains need temperature around 350 c, PH 7, olive oil as a carbon source and Incubation for 48hr. If the temperature is higher the enzyme productivity is also high. Thin layer chromatography of lipids revealed that bacillus sp has higher lipase activity than pseudomonas sp.
The present study revealed that extracellular lipase production by
Bacillus sp. and Pseudomonas sp. isolated from soil samples was found to be accelerated at optimized culture conditions such as medium pit, temperature and carbon source. From the result, it could be concluded that the medium pH of 7.0 and temperature 350c were optimum for maximizing lipase production by Bacillus sp. and Pseudomonas sp. Inferred that the optimum contain based on this study lipase activity is higher in Bacillus sp. and Pseudomonas sp. than compare other 12 strains.
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